Composite

Part:BBa_K1969024:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-14)


C terminus engineered Gpa2 protein regulated by ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI site found at 748


Design Notes

The introduced human G alpha subunit C terminal sequence is aimed for successful signal transduction.


Source

It's artificially designed.

References